百合基因组busco结果（20221116）

/public1/home/gus02/annobaihe/coreset1012/busco_genome2

[gus02@login03 busco_genome2]$ more short_summary.specific.embryophyta_odb10.busco_genome2.txt

BUSCO version is: 4.1.4

The lineage dataset is: embryophyta_odb10 (Creation date: 2020-09-10, number of species: 50, number of BUSCOs: 1614)

Summarized benchmarking in BUSCO notation for file split_genome_0928.fa

BUSCO was run in mode: genome

***** Results: *****

C:68.7%[S:65.1%,D:3.6%],F:7.2%,M:24.1%,n:1614
1109	Complete BUSCOs (C)
1051	Complete and single-copy BUSCOs (S)
58	Complete and duplicated BUSCOs (D)
117	Fragmented BUSCOs (F)
388	Missing BUSCOs (M)
1614	Total BUSCO groups searched

65Genus的转录组组装

一、65个样本合并组装结果

/public1/home/gus02/annobaihe/trans_assemble/65Genus/Ttrinity.Trinity.fasta（高强组装）

1、提取最长转录本

**方法：**根据trinity序列名字，提取最长转录本。脚本由trinity提供

/public1/home/gus02/annobaihe/trans_assemble/65Genus/submit_job_aftertrinity.pbs

input：Ttrinity.Trinity.fasta （289,844）

output：

最长转录本序列：/public1/home/gus02/annobaihe/trans_assemble/65Genus/T65_unigene.fasta (220,238)